Application
Fig1: Western blot analysis of Stat3 phosphorylation in untreated (A) and IFN-α-treated (B) SK-MEL-28 cell cultures.Application
Fig2： Western blot analysis of phosphorylated Stat3 expression in HeLa (A), IFN-α-treated HeLa (B), SK-MEL-28 (C) and IFN-α-treated Sk-MEL-28 (D) whole cell lysates.| Product Name | p-Stat3(Tyr 705) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1110034C02Rik antibody Acute Phase Response Factor antibody Acute-phase response factor antibody ADMIO antibody APRF antibody AW109958 antibody DNA binding protein APRF antibody FLJ20882 antibody HIES antibody MGC16063 antibody Signal transducer and activator of transcription 3 (acute phase response factor) antibody Signal transducer and activator of transcription 3 antibody STAT 3 antibody Stat3 antibody STAT3_HUMAN antibody |
| Product description | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
| Immunogen | A short amino acid sequence containing Tyr 705 phosphorylated Stat3 of human origin |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 1110034C02Rik antibody Acute Phase Response Factor antibody Acute-phase response factor antibody ADMIO antibody APRF antibody AW109958 antibody DNA binding protein APRF antibody FLJ20882 antibody HIES antibody MGC16063 antibody Signal transducer and activator of transcription 3 (acute phase response factor) antibody Signal transducer and activator of transcription 3 antibody STAT 3 antibody Stat3 antibody STAT3_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | p-Stat3α isoform: 91 kDa, p-Stat3β isoform: 86 kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P40763 |
|---|
Application
Fig1: Western blot analysis of Stat3 phosphorylation in untreated (A) and IFN-α-treated (B) SK-MEL-28 cell cultures.
Application
Fig2： Western blot analysis of phosphorylated Stat3 expression in HeLa (A), IFN-α-treated HeLa (B), SK-MEL-28 (C) and IFN-α-treated Sk-MEL-28 (D) whole cell lysates.| Positive Control | Hela, SK-MEL-28 |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
