Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using CDKN2A/P16 mAb against human CDKN2A/P16 (AA: 1-156) recombinant protein. (Expected MW is 19.4 kDa)Application
Figure 3:Western blot analysis using CDKN2A/P16 mAb against HEK293 (1) and CDKN2A/P16 (AA: 1-156)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Immunofluorescence analysis of Hela cells using CDKN2A/P16 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 5:Flow cytometric analysis of Hela cells using CDKN2A/P16 mouse mAb (green) and negative control (red).| Product Name | CDKN2A/P16 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A |
| Immunogen | Purified recombinant fragment of human CDKN2A/P16 (AA: 1-156) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | ARF MLM P14 P16 P19 CMM2 INK4 MTS1 TP16 CDK4I CDKN2 INK4A MTS-1 P14ARF P19ARF P16INK4 P16INK4A P16-INK4A |
|---|---|
| Molecular Weight(MW) | 16.5kDa |
| Entrez Gene | 1029 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using CDKN2A/P16 mAb against human CDKN2A/P16 (AA: 1-156) recombinant protein. (Expected MW is 19.4 kDa)
Application
Figure 3:Western blot analysis using CDKN2A/P16 mAb against HEK293 (1) and CDKN2A/P16 (AA: 1-156)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Immunofluorescence analysis of Hela cells using CDKN2A/P16 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5:Flow cytometric analysis of Hela cells using CDKN2A/P16 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
