Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using CD8a mAb against human CD8a (AA: 22–235) recombinant protein. (Expected MW is 26.4 kDa)Application
Figure 3:Western blot analysis using CD8a mAb against HEK293 (1) and CD8a (AA: 22–235)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using CD8a mouse mAb against K562 (1), Jurkat (2), and HL-60 (3) cell lysate.Application
Figure 5:Flow cytometric analysis of Hela cells using CD8a mouse mAb (green) and negative control (red).| Product Name | CD8a |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD8; p32; Leu2 |
| Immunogen | Purified recombinant fragment of human CD8a (AA: 22–235) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBFCMICC/IF |
| WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | CD8 p32 Leu2 |
|---|---|
| Molecular Weight(MW) | 25.7kDa |
| Entrez Gene | 925 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using CD8a mAb against human CD8a (AA: 22–235) recombinant protein. (Expected MW is 26.4 kDa)
Application
Figure 3:Western blot analysis using CD8a mAb against HEK293 (1) and CD8a (AA: 22–235)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using CD8a mouse mAb against K562 (1), Jurkat (2), and HL-60 (3) cell lysate.
Application
Figure 5:Flow cytometric analysis of Hela cells using CD8a mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
