Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using ATG4B mAb against human ATG4B (AA: 1-221) recombinant protein. (Expected MW is 51 kDa)Application
Figure 3:Western blot analysis using ATG4B mAb against HEK293 (1) and ATG4B (AA: 1-221)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using ATG4B mouse mAb against Hela (1), Ramos (2), and Jurkat (3) cell lysate.Application
Figure 5:Flow cytometric analysis of Hela cells using ATG4B mouse mAb (green) and negative control (red).| Product Name | ATG4B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APG4B; AUTL1 |
| Immunogen | Purified recombinant fragment of human ATG4B (AA: 1-221) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBFCMICC/IF |
| WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | APG4B AUTL1 |
|---|---|
| Molecular Weight(MW) | 44.3kDa |
| Entrez Gene | 23192 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using ATG4B mAb against human ATG4B (AA: 1-221) recombinant protein. (Expected MW is 51 kDa)
Application
Figure 3:Western blot analysis using ATG4B mAb against HEK293 (1) and ATG4B (AA: 1-221)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using ATG4B mouse mAb against Hela (1), Ramos (2), and Jurkat (3) cell lysate.
Application
Figure 5:Flow cytometric analysis of Hela cells using ATG4B mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
