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NAGR1

Catalog Number:OM639243
Human;Mouse;Rat
WB;IHC;ICC;FCM;ICC/IF
OM639243
Discount Price
OM639243-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name NAGR1
Antibody Type Primary Antibodies
Antigen Alias HNRNPM; CEAR; HNRPM; HTGR1; HNRPM4; HNRNPM4; hnRNP M
Immunogen Purified recombinant fragment of human NAGR1 (AA: 17-161) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity HumanMouseRat
Target Information
Alternative Names HNRNPM
CEAR
HNRPM
HTGR1
HNRPM4
HNRNPM4
hnRNP M
Molecular Weight(MW) 77.5kDa
Database Links
Entrez Gene 4670
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using NAGR1 mAb against human NAGR1 (AA: 17-161) recombinant protein. (Expected MW is 42.2 kDa)

Application

Figure 3:Western blot analysis using NAGR1 mAb against HEK293 (1) and NAGR1 (AA: 17-161)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Western blot analysis using NAGR1 mouse mAb against U251 (1), PC-12 (2), HepG2 (3), A549 (4), HEK293 (5), NIH/3T3 (6), and Hela (7) cell lysate.

Application

Figure 5:Immunofluorescence analysis of Hela cells using NAGR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 6:Flow cytometric analysis of Hela cells using NAGR1 mouse mAb (green) and negative control (red).

Application

Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NAGR1 mouse mAb with DAB staining.

Application

Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NAGR1 mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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