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Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Figure 2:Western blot analysis using NAGR1 mAb against human NAGR1 (AA: 17-161) recombinant protein. (Expected MW is 42.2 kDa)
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Figure 3:Western blot analysis using NAGR1 mAb against HEK293 (1) and NAGR1 (AA: 17-161)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 4:Western blot analysis using NAGR1 mouse mAb against U251 (1), PC-12 (2), HepG2 (3), A549 (4), HEK293 (5), NIH/3T3 (6), and Hela (7) cell lysate.
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Figure 5:Immunofluorescence analysis of Hela cells using NAGR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Figure 6:Flow cytometric analysis of Hela cells using NAGR1 mouse mAb (green) and negative control (red).
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Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NAGR1 mouse mAb with DAB staining.
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Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NAGR1 mouse mAb with DAB staining.