Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2: Western blot analysis using CD22 mAb against human CD22 (AA: 621-725) recombinant protein. (Expected MW is 37 kDa)Application
Figure 3: Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4: Western blot analysis using CD22 mouse mAb against L1210 (1), Hela (2), HEK293 (3), Jurkat (4), OCM-1 (5), A431 (6) and NIH/3T3 (7) cell lysate.Application
Figure 5:Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 6: Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).Application
Figure 7: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD22 mouse mAb with DAB staining.Application
Figure 8: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using CD22 mouse mAb with DAB staining.| Product Name | CD22 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SIGLEC2; SIGLEC-2 |
| Immunogen | Purified recombinant fragment of human CD22 (AA: 621-725) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | SIGLEC2 SIGLEC-2 |
|---|---|
| Molecular Weight(MW) | 95.3kDa |
| Entrez Gene | 933 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2: Western blot analysis using CD22 mAb against human CD22 (AA: 621-725) recombinant protein. (Expected MW is 37 kDa)
Application
Figure 3: Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4: Western blot analysis using CD22 mouse mAb against L1210 (1), Hela (2), HEK293 (3), Jurkat (4), OCM-1 (5), A431 (6) and NIH/3T3 (7) cell lysate.
Application
Figure 5:Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 6: Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).
Application
Figure 7: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD22 mouse mAb with DAB staining.
Application
Figure 8: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using CD22 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
