Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using RBBP7 mAb against human RBBP7 (AA: 1-200) recombinant protein. (Expected MW is 48.6 kDa)Application
Figure 3:Western blot analysis using RBBP7 mAb against HEK293 (1) and RBBP7 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using RBBP7 mouse mAb against Jurkat (1), HepG2 (2), F9 (3), C6 (4), LNCAP (5), HL-60 (6), Hela (7), and SH-SY5Y (8) cell lysate.Application
Figure 5:Immunofluorescence analysis of Hela cells using RBBP7 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Application
Figure 6:Immunofluorescence analysis of Hela cells using RBBP7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 7:Flow cytometric analysis of Hela cells using RBBP7 mouse mAb (green) and negative control (red).Application
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RBBP7 mouse mAb with DAB staining.Application
Figure 9:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using RBBP7 mouse mAb with DAB staining.| Product Name | RBBP7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RbAp46 |
| Immunogen | Purified recombinant fragment of human RBBP7 (AA: 1-200) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Alternative Names | RbAp46 |
|---|---|
| Molecular Weight(MW) | 47.8kDa |
| Entrez Gene | 5931 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using RBBP7 mAb against human RBBP7 (AA: 1-200) recombinant protein. (Expected MW is 48.6 kDa)
Application
Figure 3:Western blot analysis using RBBP7 mAb against HEK293 (1) and RBBP7 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using RBBP7 mouse mAb against Jurkat (1), HepG2 (2), F9 (3), C6 (4), LNCAP (5), HL-60 (6), Hela (7), and SH-SY5Y (8) cell lysate.
Application
Figure 5:Immunofluorescence analysis of Hela cells using RBBP7 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Application
Figure 6:Immunofluorescence analysis of Hela cells using RBBP7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 7:Flow cytometric analysis of Hela cells using RBBP7 mouse mAb (green) and negative control (red).
Application
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RBBP7 mouse mAb with DAB staining.
Application
Figure 9:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using RBBP7 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
