Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using CD130 mAb against human CD130 (AA: extra 73-231) recombinant protein. (Expected MW is 43.8 kDa)Application
Figure 3:Western blot analysis using CD130 mAb against HEK293 (1) and CD130 (AA: extra 73-231)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using CD130 mouse mAb against COS7 (1) cell lysate.Application
Figure 5:Immunofluorescence analysis of Hela cells using CD130 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 6:Flow cytometric analysis of HL-60 cells using CD130 mouse mAb (green) and negative control (red).| Product Name | CD130 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL6ST; GP130; CDW130; IL-6RB |
| Immunogen | Purified recombinant fragment of human CD130 (AA: extra 73-231) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMonkey |
| Alternative Names | IL6ST GP130 CDW130 IL-6RB |
|---|---|
| Molecular Weight(MW) | 103.5kDa |
| Entrez Gene | 3572 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using CD130 mAb against human CD130 (AA: extra 73-231) recombinant protein. (Expected MW is 43.8 kDa)
Application
Figure 3:Western blot analysis using CD130 mAb against HEK293 (1) and CD130 (AA: extra 73-231)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using CD130 mouse mAb against COS7 (1) cell lysate.
Application
Figure 5:Immunofluorescence analysis of Hela cells using CD130 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 6:Flow cytometric analysis of HL-60 cells using CD130 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
