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TBP

Catalog Number:OM639284
Human
WB;IHC;ICC;FCM;ICC/IF
OM639284
Discount Price
OM639284-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name TBP
Antibody Type Primary Antibodies
Antigen Alias HDL4; GTF2D; SCA17; TFIID; GTF2D1
Immunogen Purified recombinant fragment of human TBP (AA: 1-144) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names HDL4
GTF2D
SCA17
TFIID
GTF2D1
Molecular Weight(MW) 37.7kDa
Database Links
Entrez Gene 6908
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using TBP mAb against human TBP (AA: 1-144) recombinant protein. (Expected MW is 41.9 kDa)

Application

Figure 3:Western blot analysis using TBP mAb against HEK293 (1) and TBP (AA: 1-144)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Western blot analysis using TBP mouse mAb against NIH/3T3 (1) and SK-N-SH (2) cell lysate.

Application

Figure 5:Immunofluorescence analysis of Hela cells using TBP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 6:Flow cytometric analysis of Hela cells using TBP mouse mAb (green) and negative control (red).

Application

Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TBP mouse mAb with DAB staining.

Application

Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using TBP mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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