Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using CD299 mAb against human CD299 (AA: extra 237-399) recombinant protein. (Expected MW is 44.9 kDa)Application
Figure 3:Western blot analysis using CD299 mAb against HEK293 (1) and CD299 (AA: extra 237-399)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using CD299 mouse mAb against L-02 (1), HepG2 (2), BEL-7402 (3), SMMC-7702 (4), and HL-7702 (5) cell lysate.Application
Figure 5:Flow cytometric analysis of MOLT4 cells using CD299 mouse mAb (green) and negative control (red).| Product Name | CD299 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLEC4M; LSIGN; CD209L; L-SIGN; DCSIGNR; HP10347; DC-SIGN2; DC-SIGNR |
| Immunogen | Purified recombinant fragment of human CD299 (AA: extra 237-399) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBFCMICC/IF |
| WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | CLEC4M LSIGN CD209L L-SIGN DCSIGNR HP10347 DC-SIGN2 DC-SIGNR |
|---|---|
| Molecular Weight(MW) | 45.4kDa |
| Entrez Gene | 10332 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using CD299 mAb against human CD299 (AA: extra 237-399) recombinant protein. (Expected MW is 44.9 kDa)
Application
Figure 3:Western blot analysis using CD299 mAb against HEK293 (1) and CD299 (AA: extra 237-399)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using CD299 mouse mAb against L-02 (1), HepG2 (2), BEL-7402 (3), SMMC-7702 (4), and HL-7702 (5) cell lysate.
Application
Figure 5:Flow cytometric analysis of MOLT4 cells using CD299 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
