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EHMT2

Catalog Number:OM639343
Human
WB;IHC;ICC;FCM;ICC/IF
OM639343
Discount Price
OM639343-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name EHMT2
Antibody Type Primary Antibodies
Antigen Alias G9A; BAT8; GAT8; NG36; KMT1C; C6orf30
Immunogen Purified recombinant fragment of human EHMT2 (AA: 317-471) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names G9A
BAT8
GAT8
NG36
KMT1C
C6orf30
Molecular Weight(MW) 132.4kDa
Database Links
Entrez Gene 10919
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using EHMT2 mAb against human EHMT2 (AA: 317-471) recombinant protein. (Expected MW is 44 kDa)

Application

Figure 3:Western blot analysis using EHMT2 mAb against HEK293 (1) and EHMT2 (AA: 317-471)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Immunofluorescence analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application

Figure 5:Immunofluorescence analysis of Hela cells using EHMT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 6:Flow cytometric analysis of HL-60 cells using EHMT2 mouse mAb (green) and negative control (red).

Application

Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using EHMT2 mouse mAb with DAB staining.

Application

Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EHMT2 mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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