Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using CD127 mAb against human CD127 (AA: extra 21-239) recombinant protein. (Expected MW is 51 kDa)Application
Figure 3:Western blot analysis using CD127 mAb against HEK293 (1) and CD127 (AA: extra 21-239)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using CD127 mouse mAb against Hela (1), HL-60 (2), and U937 (3) cell lysate.Application
Figure 5:Flow cytometric analysis of HL-60 cells using CD127 mouse mAb (green) and negative control (red).| Product Name | CD127 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL7R; ILRA; IL7RA; CDW127; IL-7R-alpha |
| Immunogen | Purified recombinant fragment of human CD127 (AA: extra 21-239) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBFCMICC/IF |
| WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | IL7R ILRA IL7RA CDW127 IL-7R-alpha |
|---|---|
| Molecular Weight(MW) | 51.6kDa |
| Entrez Gene | 3575 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using CD127 mAb against human CD127 (AA: extra 21-239) recombinant protein. (Expected MW is 51 kDa)
Application
Figure 3:Western blot analysis using CD127 mAb against HEK293 (1) and CD127 (AA: extra 21-239)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using CD127 mouse mAb against Hela (1), HL-60 (2), and U937 (3) cell lysate.
Application
Figure 5:Flow cytometric analysis of HL-60 cells using CD127 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
