Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using CD53 mAb against human CD53 (AA: extra mix) recombinant protein. (Expected MW is 37 kDa)Application
Figure 3:Western blot analysis using CD53 mAb against HEK293 (1) and CD53 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using CD53 mouse mAb against Raji (1), Ramos (2), Jurkat (3), MOLT4 (4), K562 (5), HL-60 (6), THP-1 (7), and U937 (8) cell lysate.Application
Figure 5:Flow cytometric analysis of HL-60 cells using CD53 mouse mAb (green) and negative control (red).Application
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD53 mouse mAb with DAB staining.Application
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD53 mouse mAb with DAB staining.| Product Name | CD53 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MOX44; TSPAN25 |
| Immunogen | Purified recombinant fragment of human CD53 (AA: extra mix) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | MOX44 TSPAN25 |
|---|---|
| Molecular Weight(MW) | 24.3kDa |
| Entrez Gene | 963 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using CD53 mAb against human CD53 (AA: extra mix) recombinant protein. (Expected MW is 37 kDa)
Application
Figure 3:Western blot analysis using CD53 mAb against HEK293 (1) and CD53 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using CD53 mouse mAb against Raji (1), Ramos (2), Jurkat (3), MOLT4 (4), K562 (5), HL-60 (6), THP-1 (7), and U937 (8) cell lysate.
Application
Figure 5:Flow cytometric analysis of HL-60 cells using CD53 mouse mAb (green) and negative control (red).
Application
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD53 mouse mAb with DAB staining.
Application
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD53 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
