Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using CD54 mAb against human CD54 (AA: extra 28-163) recombinant protein. (Expected MW is 40.8 kDa)Application
Figure 3:Western blot analysis using CD54 mAb against HEK293 (1) and CD54 (AA: extra 28-163)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using CD54 mouse mAb against C6 (1) and HUVEC (2) cell lysate.Application
Figure 5:Flow cytometric analysis of HL-60 cells using CD54 mouse mAb (green) and negative control (red).| Product Name | CD54 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ICAM1; BB2; P3.58 |
| Immunogen | Purified recombinant fragment of human CD54 (AA: extra 28-163) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBFCMICC/IF |
| WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | ICAM1 BB2 P3.58 |
|---|---|
| Molecular Weight(MW) | 57.8kDa |
| Entrez Gene | 3383 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using CD54 mAb against human CD54 (AA: extra 28-163) recombinant protein. (Expected MW is 40.8 kDa)
Application
Figure 3:Western blot analysis using CD54 mAb against HEK293 (1) and CD54 (AA: extra 28-163)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using CD54 mouse mAb against C6 (1) and HUVEC (2) cell lysate.
Application
Figure 5:Flow cytometric analysis of HL-60 cells using CD54 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
