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CD63

Catalog Number:OM639532
Human
FCM;ICC/IF;IHC;WB
OM639532
Discount Price
OM639532-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name CD63
Antibody Type Primary Antibodies
Immunogen Purified recombinant fragment of human CD63 (AA: extra 103-203) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications FCMICC/IFIHCWB
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names MLA1
ME491
LAMP-3
OMA81H
TSPAN30
Molecular Weight(MW) 25.6kDa
Database Links
Entrez Gene 967
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using CD63 mAb against human CD63 (AA: extra 103-203) recombinant protein. (Expected MW is 37.4 kDa)

Application

Figure 3:Western blot analysis using CD63 mAb against HEK293 (1) and CD63 (AA: extra 103-203)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Flow cytometric analysis of Hela cells using CD63 mouse mAb (green) and negative control (red).

Application

Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD63 mouse mAb with DAB staining.

Application

Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD63 mouse mAb with DAB staining.

WB

(C) Exosomal surface markers CD9 and CD63 detected by western blotting.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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References(1)