Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using PD1 mAb against human PD1 (AA: extra 21-170) recombinant protein. (Expected MW is 46.6 kDa)Application
Figure 3:Immunofluorescence analysis of Hela cells using PD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 4:Flow cytometric analysis of HL-60 cells using PD1 mouse mAb (green) and negative control (red).| Product Name | PD1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDCD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1 |
| Immunogen | Purified recombinant fragment of human PD1 (AA: extra 21-170) expressed in HEK293 cells. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | PDCD1 PD-1 CD279 SLEB2 hPD-1 hPD-l hSLE1 |
|---|---|
| Molecular Weight(MW) | 31.6KD |
| Entrez Gene | 5133 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using PD1 mAb against human PD1 (AA: extra 21-170) recombinant protein. (Expected MW is 46.6 kDa)
Application
Figure 3:Immunofluorescence analysis of Hela cells using PD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 4:Flow cytometric analysis of HL-60 cells using PD1 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
