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CD227

Catalog Number:OM639569
Human
WB;IHC;FCM;ICC/IF
OM639569
Discount Price
OM639569-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name CD227
Antibody Type Primary Antibodies
Antigen Alias MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Immunogen Purified recombinant fragment of human CD227 (AA: extra 66-175) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names MUC1
EMA
MCD
PEM
PUM
KL-6
MAM6
MCKD
PEMT
H23AG
MCKD1
MUC-1
ADMCKD
ADMCKD1
CA 15-3
MUC-1/X
MUC1/ZD
MUC-1/SEC
Molecular Weight(MW) 122.1kDa
Database Links
Entrez Gene 4582
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using CD227 mAb against human CD227 (AA: extra 66-175) recombinant protein. (Expected MW is 38.5 kDa)

Application

Figure 3:Western blot analysis using CD227 mAb against HEK293 (1) and CD227 (AA: extra 66-175)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Flow cytometric analysis of Raji cells using CD227 mouse mAb (green) and negative control (red).

Application

Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD227 mouse mAb with DAB staining.

Application

Figure 6:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CD227 mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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