Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using B7H3 mAb against human B7H3 (AA: extra 29-466) recombinant protein. (Expected MW is 76.9 kDa)Application
Figure 3:Immunofluorescence analysis of Hela cells using B7H3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 4:Flow cytometric analysis of Jurkat cells using B7H3 mouse mAb (green) and negative control (red).Application
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using B7H3 mouse mAb with DAB staining.Application
Figure 6:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using B7H3 mouse mAb with DAB staining.| Product Name | B7H3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD276; B7-H3; B7RP-2; 4Ig-B7-H3 |
| Immunogen | Purified recombinant fragment of human B7H3 (AA: extra 29-466) expressed in HEK293 cells. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | CD276 B7-H3 B7RP-2 4Ig-B7-H3 |
|---|---|
| Molecular Weight(MW) | 57.2kDa |
| Entrez Gene | 80381 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using B7H3 mAb against human B7H3 (AA: extra 29-466) recombinant protein. (Expected MW is 76.9 kDa)
Application
Figure 3:Immunofluorescence analysis of Hela cells using B7H3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 4:Flow cytometric analysis of Jurkat cells using B7H3 mouse mAb (green) and negative control (red).
Application
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using B7H3 mouse mAb with DAB staining.
Application
Figure 6:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using B7H3 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
