Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using TIM3 mAb against human TIM3 (AA: extra 22-202) recombinant protein. (Expected MW is 50 kDa)Application
Figure 3:Immunofluorescence analysis of Hela cells using TIM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 4:Flow cytometric analysis of Jurkat cells using TIM3 mouse mAb (green) and negative control (red).| Product Name | TIM3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HAVCR2; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2 |
| Immunogen | Purified recombinant fragment of human TIM3 (AA: extra 22-202) expressed in HEK293 cells. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | HAVCR2 CD366 KIM-3 TIMD3 Tim-3 TIMD-3 HAVcr-2 |
|---|---|
| Molecular Weight(MW) | 33.4kDa |
| Entrez Gene | 84868 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using TIM3 mAb against human TIM3 (AA: extra 22-202) recombinant protein. (Expected MW is 50 kDa)
Application
Figure 3:Immunofluorescence analysis of Hela cells using TIM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 4:Flow cytometric analysis of Jurkat cells using TIM3 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
