Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using ELANE mAb against human ELANE (AA: 140-267) recombinant protein. (Expected MW is 39 kDa)Application
Figure 3:Western blot analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using ELANE mouse mAb against U937 (1), SPC-A-1 (2), and COS7 (3) cell lysate.Application
Figure 5:Flow cytometric analysis of Hela cells using ELANE mouse mAb (green) and negative control (red).Application
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using ELANE mouse mAb with DAB staining.| Product Name | ELANE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GE; NE; HLE; HNE; ELA2; SCN1; PMN-E |
| Immunogen | Purified recombinant fragment of human ELANE (AA: 140-267) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMonkey |
| Alternative Names | GE NE HLE HNE ELA2 SCN1 PMN-E |
|---|---|
| Molecular Weight(MW) | 28.5kDa |
| Entrez Gene | 1991 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using ELANE mAb against human ELANE (AA: 140-267) recombinant protein. (Expected MW is 39 kDa)
Application
Figure 3:Western blot analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using ELANE mouse mAb against U937 (1), SPC-A-1 (2), and COS7 (3) cell lysate.
Application
Figure 5:Flow cytometric analysis of Hela cells using ELANE mouse mAb (green) and negative control (red).
Application
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using ELANE mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
