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BCL6

Catalog Number:OM639634
Human
WB;ICC;FCM;ICC/IF
OM639634
Discount Price
OM639634-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name BCL6
Antibody Type Primary Antibodies
Antigen Alias BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
Immunogen Purified recombinant fragment of human BCL6 (AA: 147-276) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBICCFCMICC/IF
WB:1:250~1:1000
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names BCL5
LAZ3
BCL6A
ZNF51
ZBTB27
Molecular Weight(MW) 78.8kDa
Database Links
Entrez Gene 604
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using BCL6 mAb against human BCL6 (AA: 147-276) recombinant protein. (Expected MW is 40.5 kDa)

Application

Figure 3:Western blot analysis using BCL6 mAb against HEK293 (1) and BCL6 (AA: 147-276)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Immunofluorescence analysis of Hela cells using BCL6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 5:Flow cytometric analysis of K562 cells using BCL6 mouse mAb (green) and negative control (red).
Application Notes WB:1:250~1:1000
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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