Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using HTR3A mAb against human HTR3A (AA: extra 24-157) recombinant protein. (Expected MW is 41.9 kDa)Application
Figure 3:Western blot analysis using HTR3A mAb against HEK293 (1) and HTR3A (AA: extra 24-157)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Immunofluorescence analysis of Hela cells using HTR3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 5:Flow cytometric analysis of Hela cells using HTR3A mouse mAb (green) and negative control (red).Application
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using HTR3A mouse mAb with DAB staining.Application
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HTR3A mouse mAb with DAB staining.| Product Name | HTR3A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human HTR3A (AA: extra 24-157) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | FCMICCICC/IFIHCWB |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | HTR3 5HT3R 5-HT-3 5-HT3A 5-HT3R |
|---|---|
| Molecular Weight(MW) | 55.3kDa |
| Entrez Gene | 3359 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using HTR3A mAb against human HTR3A (AA: extra 24-157) recombinant protein. (Expected MW is 41.9 kDa)
Application
Figure 3:Western blot analysis using HTR3A mAb against HEK293 (1) and HTR3A (AA: extra 24-157)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Immunofluorescence analysis of Hela cells using HTR3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5:Flow cytometric analysis of Hela cells using HTR3A mouse mAb (green) and negative control (red).
Application
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using HTR3A mouse mAb with DAB staining.
Application
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HTR3A mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
