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BCL2L2

Catalog Number:OM639678
Human
WB;IHC;ICC;FCM;ICC/IF
OM639678
Discount Price
OM639678-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name BCL2L2
Antibody Type Primary Antibodies
Antigen Alias BCLW; BCL-W; PPP1R51; BCL2-L-2
Immunogen Purified recombinant fragment of human BCL2L2 (AA: 6-118) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names BCLW
BCL-W
PPP1R51
BCL2-L-2
Molecular Weight(MW) 20.7kDa
Database Links
Entrez Gene 599
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using BCL2L2 mAb against human BCL2L2 (AA: 6-118) recombinant protein. (Expected MW is 38.2 kDa)

Application

Figure 3:Western blot analysis using BCL2L2 mAb against HEK293 (1) and BCL2L2 (AA: 6-118)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Western blot analysis using BCL2L2 mouse mAb against HCT116 (1), LOVO (2), SW480 (3), and HL-60 (4) cell lysate.

Application

Figure 5:Immunofluorescence analysis of Hela cells using BCL2L2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 6:Flow cytometric analysis of Hela cells using BCL2L2 mouse mAb (green) and negative control (red).

Application

Figure 7:Flow cytometric analysis of K562 cells using BCL2L2 mouse mAb (green) and negative control (red).

Application

Figure 8:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BCL2L2 mouse mAb with DAB staining.

Application

Figure 9:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using BCL2L2 mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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