Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using ATG3 mAb against human ATG3 (AA: 1-100) recombinant protein. (Expected MW is 37.3 kDa)Application
Figure 3:Western blot analysis using ATG3 mAb against HEK293 (1) and ATG3 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using ATG3 mouse mAb against K562 (1), Hela (2), and THP-1 (3) cell lysate.Application
Figure 5:Immunofluorescence analysis of SMMC-7721 cells using ATG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 6:Flow cytometric analysis of Jurkat cells using ATG3 mouse mAb (green) and negative control (red).Application
Figure 7:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using ATG3 mouse mAb with DAB staining.Application
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATG3 mouse mAb with DAB staining.| Product Name | Mouse Monoclonal Antibody to ATG3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human ATG3 (AA: 1-100) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCMICCICC/IFIHCWB |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Purification | Affinity purified |
| Alternative Names | APG3 APG3L PC3-96 APG3-LIKE |
|---|---|
| Molecular Weight(MW) | 35.9kDa |
| Entrez Gene | 64422 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using ATG3 mAb against human ATG3 (AA: 1-100) recombinant protein. (Expected MW is 37.3 kDa)
Application
Figure 3:Western blot analysis using ATG3 mAb against HEK293 (1) and ATG3 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using ATG3 mouse mAb against K562 (1), Hela (2), and THP-1 (3) cell lysate.
Application
Figure 5:Immunofluorescence analysis of SMMC-7721 cells using ATG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 6:Flow cytometric analysis of Jurkat cells using ATG3 mouse mAb (green) and negative control (red).
Application
Figure 7:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using ATG3 mouse mAb with DAB staining.
Application
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATG3 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
