Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Immunofluorescence analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 3:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.Application
Figure 4:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.Application
Figure 5:Flow cytometric analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).| Product Name | Phospho-4E-BP1 (Ser65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I |
| Immunogen | Synthesized peptide of human Phospho-4E-BP1 (Ser65). |
| Modification | p-Ser65 |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | IHCICCFCMICC/IF |
| IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | EIF4EBP1 BP-1 4EBP1 4E-BP1 PHAS-I |
|---|---|
| Molecular Weight(MW) | 12.6kDa |
| Entrez Gene | 1978 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Immunofluorescence analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 3:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.
Application
Figure 4:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.
Application
Figure 5:Flow cytometric analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).| Application Notes | IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
