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Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Figure 2:Western blot analysis using ROP1 mAb against Toxoplasma gondii rhoptry protein ROP1 (AA: 42-183) recombinant protein. (Expected MW is 41.1 kDa)
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Figure 3:Western blot analysis using ROP1 mAb against HEK293 (1) and ROP1 (AA: 42-183)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 4:Immunofluorescence analysis of Hela cells using ROP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Figure 5:Immunofluorescence analysis of SMMC-7721 cells using ROP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Figure 6:Immunohistochemical analysis of paraffin-embedded ROP1 tissues using cervical cancer mouse mAb with DAB staining.
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Figure 7:Immunohistochemical analysis of paraffin-embedded ROP1 tissues using bladder cancer mouse mAb with DAB staining.