Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using ATF4 mAb against human ATF4 (AA: 212-351) recombinant protein. (Expected MW is 41.5 kDa)Application
Figure 3:Western blot analysis using ATF4 mAb against HEK293 (1) and ATF4 (AA: 212-351)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using ATF4 mouse mAb against K562 (1), A431 (2), Hela (3), HEK293 (4), and Ramos (5) cell lysate.Application
Figure 5:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ATF4 mouse mAb with DAB staining.Application
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATF4 mouse mAb with DAB staining.| Product Name | ATF4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CREB2; TXREB; CREB-2; TAXREB67 |
| Immunogen | Purified recombinant fragment of human ATF4 (AA: 212-351) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | CREB2 TXREB CREB-2 TAXREB67 |
|---|---|
| Molecular Weight(MW) | 38.6kDa |
| Entrez Gene | 468 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using ATF4 mAb against human ATF4 (AA: 212-351) recombinant protein. (Expected MW is 41.5 kDa)
Application
Figure 3:Western blot analysis using ATF4 mAb against HEK293 (1) and ATF4 (AA: 212-351)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using ATF4 mouse mAb against K562 (1), A431 (2), Hela (3), HEK293 (4), and Ramos (5) cell lysate.
Application
Figure 5:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ATF4 mouse mAb with DAB staining.
Application
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATF4 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
