Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using ATG2A mAb against human ATG2A (AA: 325-429) recombinant protein. (Expected MW is 37.2 kDa)Application
Figure 3:Western blot analysis using ATG2A mAb against HEK293 (1) and ATG2A (AA: 325-429)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Flow cytometric analysis of K562 cells using ATG2A mouse mAb (green) and negative control (red).Application
Figure 5:Immunofluorescence analysis of Hela cells using ATG2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)| Product Name | ATG2A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human ATG2A (AA: 325-429) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Molecular Weight(MW) | 212.8kDa |
|---|
| Entrez Gene | 23130 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using ATG2A mAb against human ATG2A (AA: 325-429) recombinant protein. (Expected MW is 37.2 kDa)
Application
Figure 3:Western blot analysis using ATG2A mAb against HEK293 (1) and ATG2A (AA: 325-429)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Flow cytometric analysis of K562 cells using ATG2A mouse mAb (green) and negative control (red).
Application
Figure 5:Immunofluorescence analysis of Hela cells using ATG2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
