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NS1

Catalog Number:OM639815
Human
WB;ICC;FCM;ICC/IF
OM639815
Discount Price
OM639815-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name NS1
Antibody Type Primary Antibodies
Antigen Alias Human parvovirus B19 isolate Vn115 NS1
Immunogen Purified recombinant fragment of human Parvovirus NS1 (AA: 1-216) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBICCFCMICC/IF
WB:1:250~1:1000
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names Human parvovirus B19 isolate Vn115 NS1
Molecular Weight(MW) 7.5kDa
Database Links
Entrez Gene DQ357065.1
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using NS1 mAb against human Parvovirus NS1 (AA: 1-216) recombinant protein. (Expected MW is 33.4 kDa)

Application

Figure 3:Western blot analysis using NS1 mAb against HEK293 (1) and NS1 (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Immunofluorescence analysis of Hela cells using NS1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application

Figure 5:Immunofluorescence analysis of Hela cells using NS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 6:Flow cytometric analysis of K562 cells using NS1 mouse mAb (green) and negative control (red).

Application

Figure 7:Flow cytometric analysis of Hela cells using NS1 mouse mAb (green) and negative control (red).
Application Notes WB:1:250~1:1000
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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