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HLA-B

Catalog Number:OM639840
Human
WB;IHC;FCM;ICC/IF
OM639840
Discount Price
OM639840-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name HLA-B
Antibody Type Primary Antibodies
Antigen Alias AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw;
Immunogen Purified recombinant fragment of human HLA-B (AA: 241-362) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names AS
HLAB
Bw-47
Bw-50
SPDA1
B-4901
B-5001
HLA-Cw
Molecular Weight(MW) 40.5kDa
Database Links
Entrez Gene 3106
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using HLA-B mAb against human HLA-B (AA: 241-362) recombinant protein. (Expected MW is 38.4 kDa)

Application

Figure 3:Western blot analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Western blot analysis using HLA-B mouse mAb against Ramos (1) and A431 (2) cell lysate.

Application

Figure 5:Flow cytometric analysis of Hela cells using HLA-B mouse mAb (green) and negative control (red).

Application

Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using HLA-B mouse mAb with DAB staining.

Application

Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HLA-B mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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