Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using IDH1 mAb against human IDH1 (AA: 156-298) recombinant protein. (Expected MW is 41.8 kDa)Application
Figure 3:Western blot analysis using IDH1 mAb against HEK293 (1) and IDH1 (AA: 156-298)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using IDH1 mouse mAb against HepG2 (1), NIH/3T3 (2), C2C12 (3), COS7 (4), and SW480 (5) cell lysate.Application
Figure 5:Immunofluorescence analysis of Hela cells using IDH1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 6:Flow cytometric analysis of Hela cells using IDH1 mouse mAb (green) and negative control (red).| Product Name | IDH1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26 |
| Immunogen | Purified recombinant fragment of human IDH1 (AA: 156-298) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseMonkey |
| Alternative Names | IDH IDP IDCD IDPC PICD HEL-216 HEL-S-26 |
|---|---|
| Molecular Weight(MW) | 46.7kDa |
| Entrez Gene | 3417 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using IDH1 mAb against human IDH1 (AA: 156-298) recombinant protein. (Expected MW is 41.8 kDa)
Application
Figure 3:Western blot analysis using IDH1 mAb against HEK293 (1) and IDH1 (AA: 156-298)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using IDH1 mouse mAb against HepG2 (1), NIH/3T3 (2), C2C12 (3), COS7 (4), and SW480 (5) cell lysate.
Application
Figure 5:Immunofluorescence analysis of Hela cells using IDH1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 6:Flow cytometric analysis of Hela cells using IDH1 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
