Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using MET mAb against human MET (AA: 743-932) recombinant protein. (Expected MW is 51 kDa)Application
Figure 3:Western blot analysis using MET mAb against HEK293 (1) and MET (AA: 743-932)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using MET mouse mAb against A549 (1), COS7 (2), Hela (3), HEK293 (4), HepG2 (5), and A431 (6) cell lysate.Application
Figure 5:Immunofluorescence analysis of Hela cells using MET mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 6:Flow cytometric analysis of MCF-7 cells using MET mouse mAb (green) and negative control (red).Application
Figure 7:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using MET mouse mAb with DAB staining.| Product Name | MET |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HGFR; AUTS9; RCCP2; c-Met; DFNB97 |
| Immunogen | Purified recombinant fragment of human MET (AA: 743-932) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMonkey |
| Alternative Names | HGFR AUTS9 RCCP2 c-Met DFNB97 |
|---|---|
| Molecular Weight(MW) | 155kDa |
| Entrez Gene | 4233 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using MET mAb against human MET (AA: 743-932) recombinant protein. (Expected MW is 51 kDa)
Application
Figure 3:Western blot analysis using MET mAb against HEK293 (1) and MET (AA: 743-932)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using MET mouse mAb against A549 (1), COS7 (2), Hela (3), HEK293 (4), HepG2 (5), and A431 (6) cell lysate.
Application
Figure 5:Immunofluorescence analysis of Hela cells using MET mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 6:Flow cytometric analysis of MCF-7 cells using MET mouse mAb (green) and negative control (red).
Application
Figure 7:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using MET mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
