Application
Figure 1:Western blot analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)Application
Figure 2:Western blot analysis using BUB1 mAb against HEK293 (1) and BUB1 (AA: 1-130)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3:Flow cytometric analysis of Hela cells using BUB1 mouse mAb (green) and negative control (red).Application
Figure 4:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)| Product Name | BUB1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BUB1A; BUB1L; Hbub1 |
| Immunogen | Purified recombinant fragment of human BUB1 (AA: 1-130) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBFCMICC/IF |
| WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | BUB1A BUB1L Hbub1 |
|---|---|
| Molecular Weight(MW) | 122.4kDa |
| Entrez Gene | 699 |
|---|
Application
Figure 1:Western blot analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)
Application
Figure 2:Western blot analysis using BUB1 mAb against HEK293 (1) and BUB1 (AA: 1-130)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3:Flow cytometric analysis of Hela cells using BUB1 mouse mAb (green) and negative control (red).
Application
Figure 4:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)| Application Notes | WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
