Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using AKT1 mAb against human AKT1 (AA: 1-150) recombinant protein. (Expected MW is 43.6 kDa)Application
Figure 3:Western blot analysis using AKT1 mAb against HEK293 (1) and AKT1 (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using AKT1 mouse mAb against A549 (1), MCF-7 (2), Hela (3), COS7 (4), C6 (5), and HL-60 (6) cell lysate.Application
Figure 5:Flow cytometric analysis of Hela cells using AKT1 mouse mAb (green) and negative control (red).Application
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using AKT1 mouse mAb with DAB staining.Application
Figure 7:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using AKT1 mouse mAb with DAB staining.| Product Name | AKT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA |
| Immunogen | Purified recombinant fragment of human AKT1 (AA: 1-150) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMonkeyRat |
| Alternative Names | AKT PKB RAC CWS6 PRKBA PKB-ALPHA RAC-ALPHA |
|---|---|
| Molecular Weight(MW) | 55.7kDa |
| Entrez Gene | 207 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using AKT1 mAb against human AKT1 (AA: 1-150) recombinant protein. (Expected MW is 43.6 kDa)
Application
Figure 3:Western blot analysis using AKT1 mAb against HEK293 (1) and AKT1 (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using AKT1 mouse mAb against A549 (1), MCF-7 (2), Hela (3), COS7 (4), C6 (5), and HL-60 (6) cell lysate.
Application
Figure 5:Flow cytometric analysis of Hela cells using AKT1 mouse mAb (green) and negative control (red).
Application
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using AKT1 mouse mAb with DAB staining.
Application
Figure 7:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using AKT1 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
