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RAD50

Catalog Number:OM639984
Human;Rat
WB;IHC;FCM;ICC/IF
OM639984
Discount Price
OM639984-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name RAD50
Antibody Type Primary Antibodies
Antigen Alias NBSLD; RAD502; hRad50
Immunogen Purified recombinant fragment of human RAD50 (AA: 228-359) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity HumanRat
Target Information
Alternative Names NBSLD
RAD502
hRad50
Molecular Weight(MW) 154kDa
Database Links
Entrez Gene 10111
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using RAD50 mAb against human RAD50 (AA: 228-359) recombinant protein. (Expected MW is 41.7 kDa)

Application

Figure 3:Western blot analysis using RAD50 mAb against HEK293 (1) and RAD50 (AA: 228-359)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Western blot analysis using RAD50 mouse mAb against C6 (1) and HepG2 (2) cell lysate.

Application

Figure 5:Flow cytometric analysis of Hela cells using RAD50 mouse mAb (green) and negative control (red).

Application

Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using RAD50 mouse mAb with DAB staining.

Application

Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using RAD50 mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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