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GFPT1

Catalog Number:OM639990
Human;Monkey;Rat
WB;ICC;FCM;ICC/IF
OM639990
Discount Price
OM639990-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name GFPT1
Antibody Type Primary Antibodies
Antigen Alias GFA; GFAT; GFPT; MSLG; CMS12; GFAT1; CMSTA1; GFAT 1; GFAT1m; GFPT1L
Immunogen Purified recombinant fragment of human GFPT1 (AA: 536-681) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBICCFCMICC/IF
WB:1:250~1:1000
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity HumanMonkeyRat
Target Information
Alternative Names GFA
GFAT
GFPT
MSLG
CMS12
GFAT1
CMSTA1
GFAT 1
GFAT1m
GFPT1L
Molecular Weight(MW) 78.8kDa
Database Links
Entrez Gene 2673
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using GFPT1 mAb against human GFPT1 (AA: 536-681) recombinant protein. (Expected MW is 42.3 kDa)

Application

Figure 3:Western blot analysis using GFPT1 mAb against HEK293 (1) and GFPT1 (AA: 536-681)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Western blot analysis using GFPT1 mouse mAb against HepG2 (1), HEK293 (2), BEL-7402 (3), SMMC-7721 (4), SK-MES-1 (5), C6 (6), and COS7 (7) cell lysate.

Application

Figure 5:Immunofluorescence analysis of Hela cells using GFPT1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 6:Flow cytometric analysis of Hela cells using GFPT1 mouse mAb (green) and negative control (red).
Application Notes WB:1:250~1:1000
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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