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PTPN6

Catalog Number:OM640020
Human
WB;ICC;FCM;ICC/IF
OM640020
Discount Price
OM640020-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name PTPN6
Antibody Type Primary Antibodies
Antigen Alias HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
Immunogen Purified recombinant fragment of human PTPN6 (AA: 243-541) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBICCFCMICC/IF
WB:1:250~1:1000
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names HCP
HCPH
SHP1
SHP-1
HPTP1C
PTP-1C
SHP-1L
SH-PTP1
Molecular Weight(MW) 67.6kDa
Database Links
Entrez Gene 5777
Application

Application

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Application

Figure 2:Western blot analysis using PTPN6 mAb against human PTPN6 (AA: 243-541) recombinant protein. (Expected MW is 60 kDa)

Application

Figure 3:Western blot analysis using PTPN6 mAb against HEK293 (1) and PTPN6 (AA: 243-541)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Immunofluorescence analysis of Hela cells using PTPN6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 5:Flow cytometric analysis of Raji cells using PTPN6 mouse mAb (green) and negative control (red).
Application Notes WB:1:250~1:1000
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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