Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 2:Western blot analysis using LEF1 mAb against human LEF1 (AA: 33-138) recombinant protein. (Expected MW is 37.1 kDa)Application
Figure 3:Western blot analysis using LEF1 mAb against HEK293 (1) and LEF1 (AA: 33-138)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using LEF1 mouse mAb against Jurkat (1), HepG2 (2), and MOLT4 (3) cell lysate.Application
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using LEF1 mouse mAb with DAB staining.Application
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using LEF1 mouse mAb with DAB staining.| Product Name | LEF1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEF-1; TCF10; TCF7L3; TCF1ALPHA |
| Immunogen | Purified recombinant fragment of human LEF1 (AA: 33-138) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | LEF-1 TCF10 TCF7L3 TCF1ALPHA |
|---|---|
| Molecular Weight(MW) | 44.2kDa |
| Entrez Gene | 51176 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Western blot analysis using LEF1 mAb against human LEF1 (AA: 33-138) recombinant protein. (Expected MW is 37.1 kDa)
Application
Figure 3:Western blot analysis using LEF1 mAb against HEK293 (1) and LEF1 (AA: 33-138)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using LEF1 mouse mAb against Jurkat (1), HepG2 (2), and MOLT4 (3) cell lysate.
Application
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using LEF1 mouse mAb with DAB staining.
Application
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using LEF1 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
