Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 2:Western blot analysis using PRDM5 mAb against human PRDM5 (AA: 1-100) recombinant protein. (Expected MW is 37.6 kDa)Application
Figure 3:Western blot analysis using PRDM5 mAb against HEK293 (1) and PRDM5 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using PRDM5 mouse mAb against HL-60 (1), NIH/3T3 (2), SW480 (3), and HEK293 (4) cell lysate.Application
Figure 5:Immunofluorescence analysis of MCF-7 cells using PRDM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 6:Flow cytometric analysis of HeLa cells using PRDM5 mouse mAb (green) and negative control (red).| Product Name | PRDM5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCS2; PFM2 |
| Immunogen | Purified recombinant fragment of human PRDM5 (AA: 1-100) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Alternative Names | BCS2 PFM2 |
|---|---|
| Molecular Weight(MW) | 73kDa |
| Entrez Gene | 11107 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Western blot analysis using PRDM5 mAb against human PRDM5 (AA: 1-100) recombinant protein. (Expected MW is 37.6 kDa)
Application
Figure 3:Western blot analysis using PRDM5 mAb against HEK293 (1) and PRDM5 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using PRDM5 mouse mAb against HL-60 (1), NIH/3T3 (2), SW480 (3), and HEK293 (4) cell lysate.
Application
Figure 5:Immunofluorescence analysis of MCF-7 cells using PRDM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 6:Flow cytometric analysis of HeLa cells using PRDM5 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
