Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 2:Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Application
Figure 3:Immunofluorescence analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 4:Flow cytometric analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).Application
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.Application
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.Application
Figure 7:Western blot analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.| Product Name | HIST2H4A(20Me) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H4; H4/n; H4F2; H4FN; FO108; HIST2H4 |
| Immunogen | Synthesized peptide of human HIST2H4A ( AA: GGAKRHRK(Me)VLRDNIQ ). |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | H4 H4/n H4F2 H4FN FO108 HIST2H4 |
|---|---|
| Molecular Weight(MW) | 11.4kDa |
| Entrez Gene | 8370 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Application
Figure 3:Immunofluorescence analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 4:Flow cytometric analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).
Application
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Application
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Application
Figure 7:Western blot analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
