Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 2:Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)Application
Figure 3:Western blot analysis using HDAC4 mAb against HEK293 (1) and HDAC4 (AA: 456-592)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Immunofluorescence analysis of HeLa cells using HDAC4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 5:Flow cytometric analysis of HeLa cells using HDAC4 mouse mAb (green) and negative control (red).| Product Name | HDAC4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A |
| Immunogen | Purified recombinant fragment of human HDAC4 (AA: 456-592) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | HD4 AHO3 BDMR HDACA HA6116 HDAC-4 HDAC-A |
|---|---|
| Molecular Weight(MW) | 119kDa |
| Entrez Gene | 9759 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)
Application
Figure 3:Western blot analysis using HDAC4 mAb against HEK293 (1) and HDAC4 (AA: 456-592)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Immunofluorescence analysis of HeLa cells using HDAC4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5:Flow cytometric analysis of HeLa cells using HDAC4 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
