Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 2:Western blot analysis using DDX39B mAb against human DDX39B (AA: 1-250) recombinant protein. (Expected MW is 54.2 kDa)Application
Figure 3:Western blot analysis using DDX39B mAb against HEK293 (1) and DDX39B (AA: 1-250)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using DDX39B mouse mAb against HepG2 (1), HepG2 (2), K562 (3), Jurkat (4), NIH/3T3 (5), MCF-7 (6), Jurkat (7), and Hek293 (8) cell lysate.Application
Figure 5:Immunofluorescence analysis of HeLa cells using DDX39B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Application
Figure 6:Immunofluorescence analysis of HeLa cells using DDX39B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 7:Immunohistochemical analysis of paraffin-embedded HeLa tissues using DDX39B mouse mAb with DAB staining.| Product Name | DDX39B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BAT1; UAP56; D6S81E |
| Immunogen | Purified recombinant fragment of human DDX39B (AA: 1-250) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Alternative Names | BAT1 UAP56 D6S81E |
|---|---|
| Molecular Weight(MW) | 49kDa |
| Entrez Gene | 7919 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Western blot analysis using DDX39B mAb against human DDX39B (AA: 1-250) recombinant protein. (Expected MW is 54.2 kDa)
Application
Figure 3:Western blot analysis using DDX39B mAb against HEK293 (1) and DDX39B (AA: 1-250)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using DDX39B mouse mAb against HepG2 (1), HepG2 (2), K562 (3), Jurkat (4), NIH/3T3 (5), MCF-7 (6), Jurkat (7), and Hek293 (8) cell lysate.
Application
Figure 5:Immunofluorescence analysis of HeLa cells using DDX39B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Application
Figure 6:Immunofluorescence analysis of HeLa cells using DDX39B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 7:Immunohistochemical analysis of paraffin-embedded HeLa tissues using DDX39B mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
