Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 2:Western blot analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)Application
Figure 3:Western blot analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using PPARA mouse mAb against HepG2 (1), HEK293 (2), COS7 (7), HepG2 (4), and Jurkat (5) cell lysate.Application
Figure 5:Flow cytometric analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).| Product Name | PPARA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPAR; NR1C1; hPPAR; PPARalpha |
| Immunogen | Purified recombinant fragment of human PPARA (AA: 1-120) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBFCMICC/IF |
| WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMonkey |
| Alternative Names | PPAR NR1C1 hPPAR PPARalpha |
|---|---|
| Molecular Weight(MW) | 52.2kDa |
| Entrez Gene | 5465 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Western blot analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)
Application
Figure 3:Western blot analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using PPARA mouse mAb against HepG2 (1), HEK293 (2), COS7 (7), HepG2 (4), and Jurkat (5) cell lysate.
Application
Figure 5:Flow cytometric analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
