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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 2:Western blot analysis using ATG16L1 mAb against human ATG16L1 (AA: 11-257) recombinant protein. (Expected MW is 55.8 kDa)
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Figure 3:Western blot analysis using ATG16L1 mAb against HEK293 (1) and ATG16L1 (AA: 11-257)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 4:Western blot analysis using ATG16L1 mouse mAb against Hela (1), Raji (2), PANC-1 (3), Jurkat (4), PC-12 (5), HepG2 (6), Hek293 (7), and NIH3T3 (8) cell lysate.
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Figure 5:Flow cytometric analysis of Hela cells using ATG16L1 mouse mAb (green) and negative control (red).