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SIRT6

Catalog Number:OM640117
Human
WB;ICC;FCM;ICC/IF
OM640117
Discount Price
OM640117-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name SIRT6
Antibody Type Primary Antibodies
Antigen Alias SIR2L6
Immunogen Purified recombinant fragment of human SIRT6 (AA: 141-250) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBICCFCMICC/IF
WB:1:250~1:1000
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names SIR2L6
Molecular Weight(MW) 39kDa
Database Links
Entrez Gene 51548
Application

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Application

Figure 2:Western blot analysis using SIRT6 mAb against human SIRT6 (AA: 141-250) recombinant protein. (Expected MW is 38 kDa)

Application

Figure 3:Western blot analysis using SIRT6 mAb against HEK293 (1) and SIRT6 (AA: 141-250)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Immunofluorescence analysis of HeLa cells using SIRT6 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application

Figure 5:Immunofluorescence analysis of HeLa cells using SIRT6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application Notes WB:1:250~1:1000
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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