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HDAC2

Catalog Number:OM640121
Human;Mouse;Monkey;Rat
WB;IHC;FCM;ICC/IF
OM640121
Discount Price
OM640121-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name HDAC2
Antibody Type Primary Antibodies
Antigen Alias HD2; RPD3; YAF1
Immunogen Purified recombinant fragment of human HDAC2 (AA: 217-327) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity HumanMouseMonkeyRat
Target Information
Alternative Names HD2
RPD3
YAF1
Molecular Weight(MW) 55.4kDa
Database Links
Entrez Gene 3066
Application

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Application

Figure 2:Western blot analysis using HDAC2 mAb against human HDAC2 (AA: 217-327) recombinant protein. (Expected MW is 38.1 kDa)

Application

Figure 3:Western blot analysis using HDAC2 mAb against HEK293 (1) and HDAC2 (AA: 217-327)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Western blot analysis using HDAC2 mouse mAb against Hela (1), Jurat (2), HepG2 (3), Hek293 (4), K562 (5), MCF-7 (6), NIH3T3 (7), COS7 (8) and PC-12 (9) cell lysate.

Application

Figure 5:Flow cytometric analysis of Hela cells using HDAC2 mouse mAb (green) and negative control (red).

Application

Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using HDAC2 mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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