Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 2:Western blot analysis using RALA mAb against human RALA (AA: 71-203) recombinant protein. (Expected MW is 41.5 kDa)Application
Figure 3:Western blot analysis using RALA mAb against HEK293 (1) and RALA (AA: 71-203)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using RALA mouse mAb against HepG2 (1), MCF-7 (2), A549 (3), K562 (4), Raji (5), MOLT4 (6), Hela (7), COS7 (8), and NIH3T3 (9) cell lysate.Application
Figure 5:Immunofluorescence analysis of Hela cells using RALA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 6:Flow cytometric analysis of MCF-7 cells using RALA mouse mAb (green) and negative control (red).Application
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using RALA mouse mAb with DAB staining.Application
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RALA mouse mAb with DAB staining.| Product Name | RALA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAL |
| Immunogen | Purified recombinant fragment of human RALA (AA: 71-203) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseMonkey |
| Alternative Names | RAL |
|---|---|
| Molecular Weight(MW) | 23.6kDa |
| Entrez Gene | 5898 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Western blot analysis using RALA mAb against human RALA (AA: 71-203) recombinant protein. (Expected MW is 41.5 kDa)
Application
Figure 3:Western blot analysis using RALA mAb against HEK293 (1) and RALA (AA: 71-203)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using RALA mouse mAb against HepG2 (1), MCF-7 (2), A549 (3), K562 (4), Raji (5), MOLT4 (6), Hela (7), COS7 (8), and NIH3T3 (9) cell lysate.
Application
Figure 5:Immunofluorescence analysis of Hela cells using RALA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 6:Flow cytometric analysis of MCF-7 cells using RALA mouse mAb (green) and negative control (red).
Application
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using RALA mouse mAb with DAB staining.
Application
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RALA mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
