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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 2:Western blot analysis using CIDEC mAb against human CIDEC (AA: 53-141) recombinant protein. (Expected MW is 36 kDa)
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Figure 3:Western blot analysis using CIDEC mAb against HEK293 (1) and CIDEC (AA: 53-141)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 4:Western blot analysis using CIDEC mouse mAb against HEK293 (1), A431 (2), and HCT116 (3) cell lysate.
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Figure 5:Immunofluorescence analysis of Hela cells using CIDEC mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Figure 6:Flow cytometric analysis of Hela cells using CIDEC mouse mAb (green) and negative control (red).