Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 2:Western blot analysis using CBX1 mAb against human CBX1 (AA: 1-185) recombinant protein. (Expected MW is 47.3 kDa)Application
Figure 3:Western blot analysis using CBX1 mAb against HEK293 (1) and CBX1 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Immunofluorescence analysis of Hela cells using CBX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 5:Flow cytometric analysis of Hela cells using CBX1 mouse mAb (green) and negative control (red).Application
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CBX1 mouse mAb with DAB staining.Application
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CBX1 mouse mAb with DAB staining.Application
Figure 8:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CBX1 mouse mAb with DAB staining.| Product Name | CBX1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta |
| Immunogen | Purified recombinant fragment of human CBX1 (AA: 1-185) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | CBX M31 MOD1 p25beta HP1-BETA HP1Hsbeta HP1Hs-beta |
|---|---|
| Molecular Weight(MW) | 21.4kDa |
| Entrez Gene | 10951 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Western blot analysis using CBX1 mAb against human CBX1 (AA: 1-185) recombinant protein. (Expected MW is 47.3 kDa)
Application
Figure 3:Western blot analysis using CBX1 mAb against HEK293 (1) and CBX1 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Immunofluorescence analysis of Hela cells using CBX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5:Flow cytometric analysis of Hela cells using CBX1 mouse mAb (green) and negative control (red).
Application
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CBX1 mouse mAb with DAB staining.
Application
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CBX1 mouse mAb with DAB staining.
Application
Figure 8:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CBX1 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
