Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 2:Western blot analysis using ANXA5 mAb against human ANXA5 (AA: 160-320) recombinant protein. (Expected MW is 44.1 kDa)Application
Figure 3:Western blot analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using ANXA5 mouse mAb against HepG2 (1), PNAC-1 (2), NIH/3T3 (3), Hela (4), MCF-7 (5), and A431 (6) cell lysate.Application
Figure 5:Immunofluorescence analysis of Hela cells using ANXA5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 6:Flow cytometric analysis of Hela cells using ANXA5 mouse mAb (green) and negative control (red).Application
Figure 7:Flow cytometric analysis of MCF-7 cells using ANXA5 mouse mAb (green) and negative control (red).| Product Name | ANXA5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PP4; ANX5; ENX2; RPRGL3; HEL-S-7 |
| Immunogen | Purified recombinant fragment of human ANXA5 (AA: 160-320) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Alternative Names | PP4 ANX5 ENX2 RPRGL3 HEL-S-7 |
|---|---|
| Molecular Weight(MW) | 36kDa |
| Entrez Gene | 308 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Western blot analysis using ANXA5 mAb against human ANXA5 (AA: 160-320) recombinant protein. (Expected MW is 44.1 kDa)
Application
Figure 3:Western blot analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using ANXA5 mouse mAb against HepG2 (1), PNAC-1 (2), NIH/3T3 (3), Hela (4), MCF-7 (5), and A431 (6) cell lysate.
Application
Figure 5:Immunofluorescence analysis of Hela cells using ANXA5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 6:Flow cytometric analysis of Hela cells using ANXA5 mouse mAb (green) and negative control (red).
Application
Figure 7:Flow cytometric analysis of MCF-7 cells using ANXA5 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody from tissue culture in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
